SAB4300670

Sigma-Aldrich

Anti-phospho-SP1 (pThr739) antibody produced in rabbit

affinity isolated antibody

Synonym(s):
Anti-TSFP1, Anti-Sp1 transcription factor
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~90 kDa

species reactivity

human, rat, mouse

concentration

1 mg/mL

application(s)

western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(T-A-Tp-P-S)

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SP1(6667)

General description

Specific protein 1 (Sp1) is a transcription regulator that has a transactivation domain at the N-terminus and a DNA-binding domain at the C-terminus, that contain three zinc fingers (ZF). It belongs to transcription factor family and is located on chromosome 12q13.

Immunogen

Peptide sequence around phosphorylation site of threonine 739 (T-A-Tp-P-S), according to the protein NP_612482.2

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Anti-phospho-SP1 (pThr739) antibody has been used in immunohistochemistry.

Biochem/physiol Actions

Specific protein 1 (Sp1) controls the up- and down-regulation of hCtr1 (human high-affinity copper transporter 1). It controls the expression of several genes that participates in differentiation, cell cycle and oncogenesis. It has the ability to bind GC/GT‐rich promoter elements with the help of C(2)H(2)‐type zinc fingers at the C‐terminal domain.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Effects of Cu(II) and cisplatin on the stability of Specific protein 1 (Sp1)-DNA binding: Insights into the regulation of copper homeostasis and platinum drug transport
Yan D, et al.
Journal of Inorganic Biochemistry (2016)
An insight into the phylogenetic history of HOX linked gene families in vertebrates
Abbasi AA and Grzeschik KH
BMC Evolutionary Biology (2007)
MiR-24 enhances radiosensitivity in nasopharyngeal carcinoma by targeting SP1
Kang M, et al.
Cancer Medicine (2016)
Regulatory mechanism of endothelin receptor B in the cerebral arteries after focal cerebral ischemia
Grell AS, et al.
PLoS ONE (2014)
Anne-Sofie Grell et al.
PloS one, 9(12), e113624-e113624 (2014-12-06)
Increased expression of endothelin receptor type B (ETBR), a vasoactive receptor, has recently been implied in the reduced cerebral blood flow and exacerbated neuronal damage after ischemia-reperfusion (I/R). The study explores the regulatory mechanisms of ETBR to identify drug targets...

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