SAB5300461

Sigma-Aldrich

Monoclonal Anti-FOXP3 antibody produced in mouse

clone 2A11G9, ascites fluid

Synonym(s):
XPID, PIDX, AIID, IPEX, JM2
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2A11G9, monoclonal

species reactivity

mouse, human

application(s)

direct ELISA: 1:10,000
immunohistochemistry: 1:200-1:1,000
indirect immunofluorescence: 1:200-1:1,000
western blot: 1:500-1:2,000

isotype

IgG1

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... Foxp3(50943)

General description

Forkhead box P3 (FOXP3) is located on human chromosome Xp11.23-Xq13.3. FOXP3 is a putative DNA-binding protein and is a member of the forkhead family. The protein is mainly expressed in the CD4+CD25+ regulatory T-cell subsets.

Immunogen

Purified recombinant fragment of human Foxp3 expressed in E.coli.
Mouse monoclonal antibody raised against Foxp3

Application

Monoclonal Anti-FOXP3 antibody has been used in immunohistochemistry (IHC).

Biochem/physiol Actions

Forkhead box P3 (FOXP3) plays an essential role in the development of CD4+CD25+ regulatory T cells and regulates immune homeostasis. It also acts as a transcriptional repressor. Polyadenylation signal mutation of the gene leads to immune dysregulation, polyendocrinopathy, enteropathy and X-linked inheritance (IPEX). Abnormalities in the gene expression are also associated with the pathogenesis of autism spectrum disorders (ASD) and unexplained recurrent spontaneous abortion (URSA).

Physical form

Ascitic fluid containing 0.03% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Correlation between protein expression of FOXP3 and level of FOXP3 promoter methylation in recurrent spontaneous abortion
Hou Wenhui, et al.
Journal of Obstetrics and Gynaecology : The Journal of the Institute of Obstetrics and Gynaecology, 42(11), 1439-1444 (2016)
Role of PIM2 in allergic asthma
Du Wei, et al.
Molecular Medicine Reports, 16(5), 7504-7512 (2017)
FOXP3 gene variations and susceptibility to autism: a case-control study
Safari, et al.
Gene, 596(6), 119-122 (2017)
Wei Du et al.
Molecular medicine reports, 16(5), 7504-7512 (2017-09-26)
T cell‑associated inflammation, particularly type 2 inflammation, has an important role in asthma pathogenesis, which is suppressed by regulatory T cells (Tregs). Proviral integration site for Moloney murine leukemia virus 2 (PIM2), a member off the serine/threonine kinase family, promotes...
Eleonora Gambineri et al.
Current opinion in rheumatology, 15(4), 430-435 (2003-06-24)
Immune dysregulation, polyendocrinopathy, enteropathy, and X-linked inheritance (IPEX) is one of a group of clinical syndromes that present with multisystem autoimmune disease suggesting a phenotype of immune dysregulation. Clinically, IPEX manifests most commonly with diarrhea, insulin-dependent diabetes mellitus, thyroid disorders...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service