MilliporeSigma
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SEP010

Millipore

Seppro® IgY14

Spin Columns

NACRES:
NA.32

Quality Level

capacity

1 mg total protein loading(14 μL of human plasma, average human protein concentration 70 mg/mL)

storage temp.

2-8°C

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General description

Seppro® IgY14 columns allow users to separate 14 highly abundant proteins (HAP) from plasma protein mixtures. The Avian polyclonal IgY (Immunoglobulin Yolk) antibodies, have a distinct, highly specific partitioning advantage over competing products, allowing the researcher to look at a much cleaner human protein mixture. Seppro® columns make further proteomics analysis into the plasma proteome much easier and provide cleaner results.

Proteins Depleted:

Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)

Application

Seppro® IgY14, spin columns (capacity: 15-20μl) is designed for use as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro® IgY14 is used to support the analysis of low abundance human serum/plasma proteins.

Legal Information

Seppro is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • S4199Seppro® Dilution buffer

  • S4324Seppro® Stripping Buffer

  • S4449Seppro® Neutralization Buffer

  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile

  • S4574Spin Column for Seppro®

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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