SHC015

Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TagFP635 Positive Control Plasmid DNA

Contains a gene encoding TagFP635, under the control of the UbC promoter

Synonym(s):
MISSION Control Vectors
MDL number:
Pricing and availability is not currently available.

product line

MISSION®

shipped in

dry ice

storage temp.

−20°C

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General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TagFP635 Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Vector is a 9226 base pair lentivirus plasmid vector that contains a gene encoding TagFP635, under the control of the UbC promoter. The UbC-TagFP635 Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TagFP635 is a trademark of Evrogen Co.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

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