SHM04

Sigma-Aldrich

MISSION® ExpressMag® Super Magnetic Plate

Increases transduction efficiency

NACRES:
NA.51
Pricing and availability is not currently available.

Quality Level

product line

MISSION®

storage temp.

room temp

General description

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

MISSION® ExpressMag® Super Magnetic Plate exerts a magnetic force used to attract magnetic nanoparticles to the surface of cells in culture. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes.
It has been used to culture rat neural stem cells for transfection of green fluorescent protein.

Caution

Do not autoclave! Clean surface with 70% isopropyl alcohol.

Legal Information

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What MISSION products have been successfully introduced into cells using ExpressMag® beads?

    MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.

  7. What is the typical fold increase in transduction efficiency when using ExpressMag beads?

    The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.

  8. What cell lines have been transduced successfully using ExpressMag Beads?

    The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.

  9. What other viruses have been successfully introduced into cells via the use of ExpressMag beads?

    Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

In vitro labeling of neural stem cells with poly-L-lysine coated super paramagnetic nanoparticles for green fluorescent protein transfection.
Albukhaty S
Iranian Biomedical Journal, 17(2), 71-76 (2013)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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