T5941

Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Synonym(s):
TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25
Pricing and availability is not currently available.

grade

BioPerformance Certified

Quality Level

assay

≥99.0% (titration)

form

crystalline powder

application(s)

cell culture | mammalian: suitable
electrophoresis: suitable

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

useful pH range

7.0 - 9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 667 mg/mL

absorption

≤0.05 at 290 at 40%

suitability

suitable for electrophoresis

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Preparation of extracts from prokaryotes.
M Cull et al.
Methods in enzymology, 182, 147-153 (1990-01-01)
Alexandar Iliev et al.
Acta histochemica, 121(1), 16-28 (2018-10-20)
The hypertrophy of the cardiac muscle is one of the most significant maladaptive mechanisms activated in response to increased workload. It is associated with histological and ultrastructural alterations, changes in the quantitative parameters and the expression of different enzymes. While...
Nadine Schrode et al.
Nature genetics, 51(10), 1475-1485 (2019-09-25)
The mechanisms by which common risk variants of small effect interact to contribute to complex genetic disorders are unclear. Here, we apply a genetic approach, using isogenic human induced pluripotent stem cells, to evaluate the effects of schizophrenia (SZ)-associated common...
Mengdie Wang et al.
Molecular biology of the cell, 30(7), 811-819 (2019-01-31)
Centrosome abnormalities are emerging hallmarks of cancer. The overproduction of centrosomes (known as centrosome amplification) has been reported in a variety of cancers and is currently being explored as a promising target for therapy. However, to understand different types of...
Karolina Hus et al.
Frontiers in plant science, 11, 614-614 (2020-06-09)
The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hybridum, describing gRNA...
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