Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:
Pricing and availability is not currently available.


BioPerformance Certified

Quality Level


≥99.0% (titration)


crystalline powder


cell culture | mammalian: suitable
electrophoresis: suitable


DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

useful pH range

7.0 - 9.0

pKa (25 °C)



150-152 °C


H2O: 667 mg/mL


≤0.05 at 290 at 40%


suitable for electrophoresis

Featured Industry

Diagnostic Assay Manufacturing

SMILES string




InChI key


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The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.


1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Preparation of extracts from prokaryotes.
M Cull et al.
Methods in enzymology, 182, 147-153 (1990-01-01)
Alexandar Iliev et al.
Acta histochemica, 121(1), 16-28 (2018-10-20)
The hypertrophy of the cardiac muscle is one of the most significant maladaptive mechanisms activated in response to increased workload. It is associated with histological and ultrastructural alterations, changes in the quantitative parameters and the expression of different enzymes. While...
Nadine Schrode et al.
Nature genetics, 51(10), 1475-1485 (2019-09-25)
The mechanisms by which common risk variants of small effect interact to contribute to complex genetic disorders are unclear. Here, we apply a genetic approach, using isogenic human induced pluripotent stem cells, to evaluate the effects of schizophrenia (SZ)-associated common...
Mengdie Wang et al.
Molecular biology of the cell, 30(7), 811-819 (2019-01-31)
Centrosome abnormalities are emerging hallmarks of cancer. The overproduction of centrosomes (known as centrosome amplification) has been reported in a variety of cancers and is currently being explored as a promising target for therapy. However, to understand different types of...
Karolina Hus et al.
Frontiers in plant science, 11, 614-614 (2020-06-09)
The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hybridum, describing gRNA...
Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography
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Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
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<i>In Situ</i> Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
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