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Thermolysin from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

Quality Level

biological source

Geobacillus stearothermophilus

product line




specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by



cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


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A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.


25 mg in poly bottle
100, 250 mg in glass bottle

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Allen F Ryan et al.
Frontiers in genetics, 11, 358-358 (2020-05-01)
Single-cell transcriptomics was used to profile cells of the normal murine middle ear. Clustering analysis of 6770 transcriptomes identified 17 cell clusters corresponding to distinct cell types: five epithelial, three stromal, three lymphocyte, two monocyte, two endothelial, one pericyte and...
Yuichi Kawasaki et al.
Journal of biochemistry, 153(1), 85-92 (2012-10-23)
The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stability [Yasukawa, K. and Inouye, K. (2007) Improving the activity and stability of thermolysin by site-directed mutagenesis. Biochim. Biophys. Acta 1774, 1281-1288] and...
Simon Cross et al.
Journal of chemical information and modeling, 52(10), 2587-2598 (2012-09-14)
Pharmacophore elucidation approaches are routinely used in drug discovery, primarily with the aim of determining the three-dimensional arrangement of common features shared by ligands interacting at the site of interest; these features can then be used to investigate the structure-activity...
Yuchen Gu et al.
Food chemistry, 141(3), 2682-2690 (2013-07-23)
The importance of soy products in reducing the risk of cardiovascular disease is well documented. Our previous computation study has indicated the presence of several potent ACE inhibitory peptides within soybean proteins which needs to be identified. The aim of...
Raymond G Sierra et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1584-1587 (2012-10-24)
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond...

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