UN 2821 6.1 / PGIII
174.2 °F - closed cup
79 °C - closed cup
We recommend harvesting the cells in PBS with a rubber policeman/cell scraper, transfer to an eppendorf tube, and proceed with the RNA isolation by removing the PBS from the cell pellet and re-suspending the pellet in the Tri Reagent.
What is important is to keep the ratio the same. For example, we recommend adding 200 μL of chloroform and 500 μL of isopropanol when using 1 ml of Tri Reagent. Therefore, 100 μL of chloroform and 250 μL of isopropanol would be added if using 500 μL of Tri Reagent. Similarly, 300 μL of chloroform and 750 μL of isopropanol would be added to if using 1.5 ml of Tri Reagent.
The expiration date for T9424 has not been established. However, based on our storage guidelines, it should be usable for 1 year from the date of receipt.
No, we recommend using T3809 Tri Reagent BD for work in blood samples.
For chloroform and isopropanol we recommend C2432 and I9616, respectively.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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