TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

usage

sufficient for ~200 assays

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principle

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Signal Word

Danger

Target Organs

Respiratory system

RIDADR

UN 3316 9

Certificate of Analysis
Certificate of Origin
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Aberrant glycosylation of alphavbeta3 integrin is associated with melanoma progression
Pochec E, et al.
Anticancer Research, 35(4), 2093-2103 (2015)
Alberto Elmi et al.
Animals : an open access journal from MDPI, 10(3) (2020-03-12)
The roe deer (Capreolus capreolus) represents a spontaneous model of testicular inactivation: During winter, bucks show a suspension of spermatogenesis that starts again in spring and peaks during the breeding season (July-August). The underlying mechanisms to the regulation of the...
Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress
Bernardini C, et al.
Cell Stress & Chaperones, 15(2), 219-224 (2010)
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive...
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