WGA3

Sigma-Aldrich

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

application(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP.

Features and Benefits

  • Starting material: 10 ng
  • Expected yield: 5 - 10 μg
  • Time required: ~1.5 hours
  • Provides a method to accurately amplify whole genome amplified DNA
  • Successive reamplification with WGA-3 provides DNA with little genetic bias when compared to the original genome
  • Maintains representation of the entire genome through subsequent reamplifications
  • Preserves precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500-fold)
  • Minimal allele drop out maintained through five successive cycles
  • Increases the number of downstream assays and allows more information to be gathered on that sample
  • Provides the opportunity to re-amplify the WGA DNA without having to purchase an entire WGA kit

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mL

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Yun Xia et al.
Frontiers in genetics, 10, 1364-1364 (2020-02-11)
Chromosomal rearrangements have long fascinated evolutionary biologists for being widely implicated in causing genetic differentiation. Suppressed recombination has been demonstrated in various species with inversion; however, there is controversy over whether such recombination suppression would facilitate divergence in reciprocal translocation...
Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and...
A multiplex PCR predictor. for aCGH success of FFPE samples.
Van Beers E.H., et al.
British Journal of Cancer, 94, 333-337 (2005)
Rita Scardino et al.
Genes, 11(4) (2020-04-05)
The history of each human chromosome can be studied through comparative cytogenetic approaches in mammals which permit the identification of human chromosomal homologies and rearrangements between species. Comparative banding, chromosome painting, Bacterial Artificial Chromosome (BAC) mapping and genome data permit...
Churamani Khanal et al.
Plant disease, 103(8), 1835-1842 (2019-06-14)
This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using...
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