WGA4

Sigma-Aldrich

GenomePlex® Single Cell Whole Genome Amplification Kit

Amplify genome of a single cell

EC Number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

application(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

GenomePlex Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Application

GenomePlex® Single Cell Whole Genome Amplification Kit has been used:
  • to amplify the isolated DNA
  • in whole genome amplification (WGA)
  • to amplify the microdissected DNAs

Features and Benefits

  • Starting material: 1 cell
  • Expected yield: 5 - 10 μg
  • Time required: ~4 hours
  • Abundant DNA yield within four hours
  • Complete representation of the entire genome with minimal allele bias
  • Includes all of the reagents necessary for cell lysis and subsequent whole genome amplification
  • Designed to amplify the genome of a single cell from any source including lymphocytes, cancer cells, epithelial cells, fibroblast, amniotic cells, polycarbonate fixed cells, and plant cells

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • L104310x Single Cell Lysis & Fragmentation Buffer

  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL

  • W4502Water, Nuclease-Free Water, for Molecular Biology

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can the amplified Omniplex products resulting from the use of Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used for TA cloning?

    Yes.  Please ensure that a final extension of 7-30 minutes at 72°C is included after amplification if performing subsequent TA cloning reactions.

  4. Can Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used with purified DNA?

    Yes. The kit is designed to amplify the small amount of DNA that is present in a single cell (3-5 pg). It will also work with small amounts of purified DNA; we recommend less than 100 pg.

  5. What kinds of cells can be used as starting materials with Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit?

    The cell lysis method involves treatment with proteinase K and heat. Most cells will be lysed under these conditions.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nicholas Navin et al.
Nature, 472(7341), 90-94 (2011-03-15)
Genomic analysis provides insights into the role of copy number variation in disease, but most methods are not designed to resolve mixed populations of cells. In tumours, where genetic heterogeneity is common, very important information may be lost that would...
Timour Baslan et al.
eLife, 9 (2020-05-14)
Copy number alterations (CNAs) play an important role in molding the genomes of breast cancers and have been shown to be clinically useful for prognostic and therapeutic purposes. However, our knowledge of intra-tumoral genetic heterogeneity of this important class of...
Jochen B Geigl et al.
Nature protocols, 2(12), 3173-3184 (2007-12-15)
A comprehensive genomic analysis of single cells is instrumental for numerous applications in tumor genetics, clinical diagnostics and forensic analyses. Here, we provide a protocol for single-cell isolation and whole genome amplification, which includes the following stages: preparation of single-cell...
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained...
Heike Fiegler et al.
Nucleic acids research, 35(3), e15-e15 (2006-12-21)
Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount...
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GenomePlex<sup>®</sup> is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
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