X4126

Sigma-Aldrich

Xylene Cyanol FF

for molecular biology, BioReagent

Synonym(s):
XC, Acid blue 147, Cyanol FF
Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
Molecular Weight:
538.61
Colour Index Number:
42135
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52
Pricing and availability is not currently available.

Quality Level

grade

for molecular biology

product line

BioReagent

form

powder

solubility

H2O: complete 1 mg/mL, clear, blue

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

InChI key

VVLFAAMTGMGYBS-KRQUPCAFSA-M

Looking for similar products? Visit Product Comparison Guide

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis
  • gel-loading dye
  • formamide RNA loading dye
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Packaging

10 g in glass bottle

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
<BIG>Afonin KA, et al.</BIG>
Nature Protocols, 6, 2022-2022 (2011)
Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
<BIG>Thiel WH, et al.</BIG>
Methods in Molecular Biology, 187-199 (2015)
Joseph Russo et al.
Methods (San Diego, Calif.), 155, 116-123 (2018-12-07)
Both RNA synthesis and decay must be balanced within a cell to achieve proper gene expression. Additionally, modulation of RNA decay specifically offers the cell an opportunity to rapidly reshape the transcriptome in response to specific stimuli or cues. Therefore...
Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
<BIG>Ota M, et al.</BIG>
Nature Protocols, 2857-2857 (2007)
P N Hengen
Trends in biochemical sciences, 20(5), 202-203 (1995-05-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column describes how to make an electrophoresis system from common household items. For details on how to partake...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.