XNAPE

Sigma-Aldrich

REDExtract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 500 amplifications

NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

usage

sufficient for 100 extractions
sufficient for 500 amplifications

feature

hotstart

color

red

shipped in

wet ice

storage temp.

−20°C

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General description

The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp Plant PCR Kit has been used to extract genomic DNA from plants and to perform polymerase chain reaction.

Features and Benefits

  • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
  • Compatible with high-throughput requirements for genetic analysis of plants
  • Extract stable at 4 °C for at least 6 months (Fig. 3)

Components

Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Mark A Jones et al.
Journal of experimental botany, 58(6), 1261-1270 (2007-02-16)
Reactive oxygen species (ROS) production by an NADPH oxidase (NOX) encoded by AtrbohC/RHD2 is required for root hair growth in Arabidopsis thaliana. ROP (RHO of plants) GTPases are also required for normal root hair growth and have been proposed to...
Ralf G Kynast et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(26), 9921-9926 (2004-06-16)
We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is...
Yuri Jorge Peña Ramírez et al.
Clinical and vaccine immunology : CVI, 14(6), 685-692 (2007-04-27)
The human immunodeficiency virus type 1 (HIV-1) Tat protein is considered a potential candidate vaccine antigen. In an effort to design a strategy for noninvasive vaccination against HIV-1, we developed transgenic tomatoes expressing the Tat protein. Two independent plants testing...
Anna Stöger et al.
Journal of microbiological methods, 58(2), 281-284 (2004-07-06)
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming...
Avinash C Srivastava et al.
Plant physiology, 148(1), 200-211 (2008-07-25)
AtSUC2 (At1g22710) encodes a phloem-localized sucrose (Suc)/H(+) symporter necessary for efficient Suc transport from source tissues to sink tissues in Arabidopsis (Arabidopsis thaliana). AtSUC2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading...
Articles
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.
Read More
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Read More
Protocols
The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves
Read More

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