REDExtract-N-Amp Tissue PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

EC Number:

Note: This product number has been replaced by the identical product XNAT


sufficient for 10 amplifications
sufficient for 10 extractions

shipped in

wet ice

storage temp.


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REDExtract-N-Amp tissue PCR kit has been for:
  • DNA extraction
  • genotyping
  • polymerase chain reaction (PCR)

Features and Benefits

  • Perfect for quick genomic DNA isolation for genotyping
  • Single-step extraction of genomic DNA for PCR in 15 minutes
  • PCR ReadyMix, specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4 oC for at least 6 months


Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.


The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC


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NONH for all modes of transport

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Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
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The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
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The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
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