53814-U

Supelco

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Quality Level

Agency/Method

suitable for USP L1

feature

endcapped

mfr. no.

Ascentis

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

application(s)

HPLC: suitable
UHPLC: suitable

L × I.D.

10 cm × 3 mm

impurities

<5 ppm metals

matrix

Fused-Core particle platform

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2 - 9

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the
Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is special about Ascentis Express?

    Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  4. When are additional phases expected?

    Additional phases are being developed.  The best way to track new products is to visit Ascentis Express for the latest updates.

  5. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

  6. Can I use Ascentis Express on any type of HPLC system?

    Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

  7. Can I use Ascentis Express on a UHPLC system?

    Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within...
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human...
Virginia Brighenti et al.
Journal of pharmaceutical and biomedical analysis, 143, 228-236 (2017-06-14)
The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed...
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)...
Ahmed Abrahim et al.
Journal of pharmaceutical and biomedical analysis, 51(1), 131-137 (2009-09-18)
Fused-core silica stationary phases represent a key technological advancement in the arena of fast HPLC separations. These phases are made by fusing a 0.5 microm porous silica layer onto 1.7 microm nonporous silica cores. The reduced intra-particle flow path of...
Articles
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
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Sigma-Aldrich presents an article on serum lipidomics profiling using an Ascentis Express Fused-Core C18 column.
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Ascentis® Express C18 is the first choice when starting a new separation method.
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Protocols
Extraction and Analysis of Neonicotinoid Pesticides from Flower Blossoms Using Supel<sup>™</sup> QuE and Ascentis<sup>®</sup> Express
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LC/MS/MS Analysis of Neonicotinoid Pesticides in Dandelion Blossoms on Ascentis® Express C18 after Dispersive SPE (QuEChERS) using Supel™ QuE
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Phenolic Antioxidants are added to polyers for controlling their degradation process. Irgafos 168, Irganox 1010, Ethanox 330, Irganox 1076, BHT, and Irgafos 168 are often used as antioxidants to prevent the degradation of polypropylene polymer formulations. This HPLC method is for the quantitation of an additive or mix of additives in a polymeric material.
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On Friday, April 27, 2018, the European Union decided to ban the use of three neonicotinoid insecticides from use on field crops, having deemed them dangerous to bees. This application demonstrates the analysis of these banned compounds and others from dandelion blossoms using QuEChERS and LC-MS.
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