Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

Pricing and availability is not currently available.

Quality Level


suitable for USP L60



mfr. no.


extent of labeling

19.5% Carbon loading


≤70 °C temp. range
400 bar pressure (5801 psi)


HPLC: suitable

L × I.D.

5 cm × 4.6 mm

surface coverage

2.7 μmol/m2


<5 ppm metals


silica gel high purity, spherical

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

100 Å

surface area

450 m2/g

operating pH range

2 - 8

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three...
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express...
Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, consumer products and as environmental pollutants dictates the need for a reliable analytical method. Ascentis HPLC columns, by virtue of their effi ciency and choices in stationary phase selectivity, are ideally suited for the analysis of fl uorinated compounds.
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