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565324-U

Supelco

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.

Quality Level

Agency/Method

suitable for USP L60

feature

endcapped

mfr. no.

Ascentis

extent of labeling

19.5% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

application(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface coverage

2.7 μmol/m2

impurities

<5 ppm metals

matrix

silica gel high purity, spherical

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

100 Å

surface area

450 m2/g

operating pH range

2 - 8

Featured Industry

Food and Beverages

separation technique

reversed phase

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three...
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express...
Articles
Reference Standards for Analyzing Polyphenol Catechins
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Protocols
Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases
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Separation of (L)-Dehydroascorbic acid; L-Ascorbic acid 20 μg/mL
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Separation of Resorcinol 50 mg/mL; Pyrocatechol; 2-Methylresorcinol; 4-Methylcatechol; 2,5-Dimethylresorcinol 50 mg/mL; 3-Methylcatechol 50 mg/mL; 4-Nitrocatechol 50 mg/mL
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HPLC Analysis of Carotene Compounds on Ascentis® RP-Amide
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