Ascentis® ES-Cyano HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Pricing and availability is not currently available.

Quality Level


suitable for USP L10



mfr. no.


extent of labeling

10% Carbon loading


≤70 °C temp. range
400 bar pressure (5801 psi)


HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface coverage

2.5 μmol/m2


<5 ppm metals


silica gel high purity, spherical

matrix active group

cyano phase

particle size

5 μm

pore size

100 Å

surface area

450 m2/g

operating pH

1 - 8

Featured Industry

Food and Beverages

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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General description

Extra stable for low pH mobile phases due to sterically protected phase.

Useful for selectivity in the reversed-phase mode, including π π and dipole-dipole interacting compounds. Can also be used HILIC mode and normal phase chromatography.

Features and Benefits

  • Enhanced stability at low pH
  • Operates in reversed-phase, HILIC, and normal phase modes of chromatography
  • Low MS bleed
  • 100% aqueous compatible
  • Available as 3 μm and 5 μm particles
  • Particle composition: Type B silica gel
  • Particle shape: Spherical

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Certificate of Analysis
Certificate of Origin
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a...
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar...
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl...
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions...

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