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822854

TSKgel® SuperSW mAb HR HPLC Column

phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size

Synonym(s):
TSKgel Size Exclusion (SW-Type) HPLC Column
Pricing and availability is not currently available.

mfr. no.

Tosoh SuperSW mAb HR, 22854

parameter

≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure

application(s)

HPLC: suitable

L × I.D.

30 m × 7.8 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

4 μm

pore size

250 Å pore size

operating pH

2.5 - 7.5

suitability

suitable for L20 per USP
suitable for L59 per USP

separation technique

size exclusion (SEC)

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General description

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.

Application

TSKgel® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).

Physical form

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation
Certificate of Analysis
Certificate of Origin
Alexander Rau et al.
Molecular cancer therapeutics, 19(7), 1474-1485 (2020-05-21)
The frequent activation of HER3 signaling as a resistance mechanism to EGFR-targeted therapy has motivated the development of combination therapies that block more than one receptor tyrosine kinase. Here, we have developed a novel tetravalent, bispecific single-chain diabody-Fc fusion protein...
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Articles
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
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Protocols
Calibration Curves for TSKgel® SW mAb Columns
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