00713

Sigma-Aldrich

Atto 514-Biotin

suitable for fluorescence

NACRES:
NA.32
Pricing and availability is not currently available.

assay

≥80.0% (HPCE)

Quality Level

mol wt

Mw 1178 g/mol

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 514 is a new hydrophilic fluorescent label with excellent water solubility. The dye exhibits strong absorption, high fluorescence quantum yield and exceptional thermal and photo-stability. Thus Atto 514 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is the 514 nm line of the Argon-Ion laser.
Biotin conjugates can be used in applications like ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

find more information
here

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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