28923

Sigma-Aldrich

Atto 550-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

eCl@ss:
34058011

Quality Level

product line

BioReagent

assay

≥90% (HPCE)

fluorescence

λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550-Biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood...
Rula Masoud et al.
ACS nano, 6(7), 6272-6283 (2012-06-06)
We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy...
Robert H Meltzer et al.
Lab on a chip, 11(5), 863-873 (2011-01-21)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires different detection strategies. Here, we...
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