41784

Sigma-Aldrich

Atto 647 maleimide

BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥90% (coupling to thiols)

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 647 belongs to a new generation of fluorescent labels for the red spectral region with high molecular absorption (120.000) and quantum yield (0.20) as well as sufficient stoke′s shift (excitation maximum 645 nm, emission maximum 669 nm). Atto 647 is characterized by a high photostability. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Atto The dye is very stable under physiological pH-values and in buffers up to pH 8.5. However, it slowly degrades at higher pH. Atto 647 maleimide shows excellent solubility in polar solvents like DMF, DMSO or acetonitrile. After coupling to a substrate the dye moiety is electrically neutral.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Andreas Bruckbauer et al.
Biophysical journal, 93(9), 3120-3131 (2007-07-17)
We have developed a new method, using a nanopipette, for controlled voltage-driven delivery of individual fluorescently labeled probe molecules to the plasma membrane which we used for single-molecule fluorescence tracking (SMT). The advantages of the method are 1), application of...
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their...
Jean-Manuel Segura et al.
The Journal of biological chemistry, 283(35), 24254-24263 (2008-06-27)
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the...
Nicole Marmé et al.
Bioconjugate chemistry, 14(6), 1133-1139 (2003-11-20)
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655...
Sandeep Kumar Vashist
Analytical biochemistry, 421(1), 336-338 (2011-11-19)
A surface plasmon resonance (SPR)-based procedure was developed to determine the effect of antibody modifications on its biomolecular binding behavior. Mouse immunoglobulin G (IgG) was immobilized on a protein A-functionalized gold-coated SPR chip. Goat anti-mouse IgG and its various commercially...

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