51797

Sigma-Aldrich

Atto MB2 maleimide

Methylene Blue derivative

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

λmax

658 nm in ethanol (lit.)
667 nm in 0.1 M phosphate pH 7.0

storage temp.

−20°C

Application

ATTO MB2 is a derivative of the well-known redox dye Methylene Blue. The dye can be reversibly reduced to the colorless leuko form. Upon oxidation the dye recovers and the absorption is fully restored. In common with most ATTO-labels the dye shows a high extinction coefficient. The dye is moderately hydrophilic. ATTO MB2 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides react more selectively with thiols. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Matthew Endara et al.
Advances in skin & wound care, 25(12), 549-555 (2012-11-16)
Chronic wounds are typically halted in the inflammatory stage of wound healing secondary to a prolonged inflammatory response of the body to bacterial colonization, as planktonic bacteria and biofilm and senescent cells present at the wound's edges. Surgical debridement of...
Methylene blue as the safest blue dye for sentinel node mapping: emphasis on anaphylaxis reaction.
Sadeghi Ramin et al.
Acta oncologica (Stockholm, Sweden), 50(5), 729-731 (2011-03-19)
Aleksandra K Bruchey et al.
American journal of pharmacology and toxicology, 3(1), 72-79 (2008-01-01)
The goals of this review were to identify methylene blue (MB) as a compound that follows hormetic behavior for a wide range of effects and to address the question of what is unique about MB that could account for its...

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