55819

Sigma-Aldrich

Atto 680-Biotin

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Synonym(s):
Biotin-Atto 680
MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

assay

≥90.0% (HPCE)

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

InChI

1S/C42H57N7O7S2/c1-4-48-18-10-12-27-20-30-35(22-33(27)48)56-36-23-34-29(21-31(36)45-30)28(26-58(53,54)55)24-42(2,3)49(34)19-11-15-39(51)44-17-9-5-8-16-43-38(50)14-7-6-13-37-40-32(25-57-37)46-41(52)47-40/h20-24,32,37,40H,4-19,25-26H2,1-3H3,(H4-,43,44,46,47,50,51,52,53,54,55)/t32-,37?,40-/m0/s1

InChI key

SMZVEGAKSTTZDO-QYIPWQSGSA-N

Application

Atto 680 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, good fluorescence quantum yield, excellent thermal and photo-stability, and very little triplet formation. Atto 680 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Nicole Marmé et al.
Bioconjugate chemistry, 14(6), 1133-1139 (2003-11-20)
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655...
Philip Tinnefeld et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 6(2), 217-222 (2005-03-09)
Light-harvesting complexes, one of nature's supreme examples of nanoscale engineering, have inspired researchers to construct molecular optical devices, such as photonic wires, which are optimised for efficient transfer of excited-state energy over large distances. The control parameters for the design...
Mike Heilemann et al.
Journal of the American Chemical Society, 126(21), 6514-6515 (2004-05-27)
We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various...
Irene Drude et al.
Biochemical and biophysical research communications, 363(1), 24-29 (2007-09-11)
Over the past two decades, the structure and mechanism of catalytic RNA have been extensively studied; now ribozymes are understood well enough to turn them into useful tools. After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides...
Slawomir Gwiazda et al.
Biochimie, 94(7), 1457-1463 (2012-04-03)
The RNA-world-theory is one possible explanation of how life on earth has evolved. In this context it is of high interest to search for molecular systems, capable of self-organization into structures with increasing complexity. We have engineered a simple catalytic...

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