69616

Sigma-Aldrich

Atto 725-Biotin

suitable for fluorescence, ≥90.0% (HPCE)

NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

assay

≥90.0% (HPCE)

fluorescence

λex 729 nm; λem 752 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 725 belongs to a new generation of fluorescent labels for the near infrared spectral region. The dye is designed
for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the dye are
strong absorption and good fluorescence as well as excellent thermal and photo-stability. Atto 725 is a cationic dye.
After coupling to a substrate the dye carries a net electrical charge of +1.
Atto 725 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH.
If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the
reaction.
Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ-hybridization, flow
cytometry and others, to identify streptavidin, avidin or extravidin-conjugates.

find more information
here

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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