72978

Sigma-Aldrich

Atto 620-Biotin

suitable for fluorescence, ≥90.0% (HPCE)

NACRES:
NA.32
Pricing and availability is not currently available.

assay

≥90.0% (HPCE)

fluorescence

λex 619 nm; λem 643 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 620 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labelling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, temperature dependent fluorescence, high thermal and photo-stability, good water
solubility, and very little triplet formation. Atto 620 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.
Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ-hybridization, flow cytometry and others, to identify streptavidin, avidin or extravidin-conjugates.

find more information
here

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Inorganic chemistry, 51(21), 11220-11222 (2012-10-18)
Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF...
Maria Strianese et al.
Journal of inorganic biochemistry, 104(6), 619-624 (2010-03-23)
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Peter P T Surawski et al.
Molecular bioSystems, 4(7), 774-778 (2008-06-20)
The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the...
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This study presents the use of flow cytometry as a high-throughput quantifiable technique to study multicomponent adsorption interactions between proteins and surfaces. Flow cytometry offers the advantage of high-throughput analysis of multiple parameters on a very small sampling scale. This...

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