87784

Sigma-Aldrich

Atto Thio12 Biotin

Pricing and availability is not currently available.

Quality Level

fluorescence

λex 579 nm; λem 609 nm

storage temp.

−20°C

General description

Atto Thio12 Biotin is a new label closely related to the well-known rhodamines. The dye can be used for labeling of DNA, RNA or proteins. The characteristic feature of the label is high yield of triplet formation.The dye is moderately hydrophilic. Atto Thio12 Biotin is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ-hybridization, flow cytometry and others, to identify streptavidin, avidin or extravidin-conjugates.

RIDADR

NONH for all modes of transport

WGK Germany

3

Andriy Chmyrov et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 7(11), 1378-1385 (2008-10-30)
Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus...

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