94875

Sigma-Aldrich

Atto 680

BioReagent, suitable for fluorescence, ≥90% (HPLC)

MDL number:
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.

product line

BioReagent

assay

≥90% (HPLC)

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

InChI

1S/C27H31N3O6S/c1-4-29-9-5-7-17-11-20-24(13-22(17)29)36-25-14-23-19(12-21(25)28-20)18(16-37(33,34)35)15-27(2,3)30(23)10-6-8-26(31)32/h11-15H,4-10,16H2,1-3H3,(H-,31,32,33,34,35)

InChI key

MHHMNDJIDRZZNT-UHFFFAOYSA-N

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General description

Find more information here

Application

Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 680 shows a molar extinction of 120,000 and QY of 30% in water (40% in ethanol). Decay time is 1.8 ns.
Atto 680 has been used as a dye to study the fluorescence quantum yields of bioanalytically relevant red dyes.

Other Notes

New red absorbing fluorescent dye as a biophysical probe for binding interactions; used as fluorescence tracer in spray diagnostics.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Integrating sphere setup for the traceable measurement of absolute photoluminescence quantum yields in the near infrared
Wu?rth C, et al.
Analytical Chemistry, 84(3), 1345-1352 (2012)
Dual laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions - evaluation of new red fluorescent dyes.
K.M. Swift et al.
Proc. SPIE: Int. Soc. Opt. Eng., 4252, 47-47 (2001)
Spray diagnostics using an all-solid-state Nd:YAIO3 laser and fluorescence tracers in commercial gasoline and diesel fuels.
I. Duwell et al.
Applied Physics B: Lasers and Optics, 79, 249-254 (2004)
Volker Buschmann et al.
Bioconjugate chemistry, 14(1), 195-204 (2003-01-16)
The spectroscopic characteristics (absorption, emission, and fluorescence lifetime) of 13 commercially available red-absorbing fluorescent dyes were studied under a variety of conditions. The dyes included in this study are Alexa647, ATTO655, ATTO680, Bodipy630/650, Cy5, Cy5.5, DiD, DY-630, DY-635, DY-640, DY-650...
Philip Tinnefeld et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 6(2), 217-222 (2005-03-09)
Light-harvesting complexes, one of nature's supreme examples of nanoscale engineering, have inspired researchers to construct molecular optical devices, such as photonic wires, which are optimised for efficient transfer of excited-state energy over large distances. The control parameters for the design...

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