Nile Blue A

certified by the Biological Stain Commission, Dye content ≥70 %

Basic Blue 12, Nile blue sulfate
Linear Formula:
2C20H20N3O · SO4
CAS Number:
Molecular Weight:
Colour Index Number:
EC Number:
MDL number:
PubChem Substance ID:


certified by the Biological Stain Commission


Dye content, ≥70%


>300 °C (dec.) (lit.)


633 nm

SMILES string




InChI key


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Features and Benefits

Used in the preparation of an amperometric glucose sensor.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Jason D Slinker et al.
Nature chemistry, 3(3), 228-233 (2011-02-22)
Molecular wires show promise in nanoscale electronics, but the synthesis of uniform, long conductive molecules is a significant challenge. Deoxyribonucleic acid (DNA) of precise length, by contrast, is synthesized easily, but its conductivity over the distances required for nanoscale devices...
A Ghanadzadeh Gilani et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 83(1), 100-105 (2011-09-06)
The visible absorption spectra of two oxazine dyes, brilliant cresyl blue and nile blue, in aqueous and aqueous urea solutions (0.01-1 M) were studied as a function of the dye concentration. Due to structural similarity of the investigated dyes, their...
Julie Dutet et al.
Pharmaceutical research, 29(12), 3464-3474 (2012-08-08)
To estimate the in vitro ungual penetration depth of sodium fluorescein and nile blue chloride by laser scanning confocal microscopy. The depth, uniformity and pathways of penetration of both markers into human nail during passive and iontophoretic experiments were investigated....
Zhiping Cui et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 83(1), 1-7 (2011-09-29)
A dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect chondroitin sulfate (CS) with nile blue sulfate (NBS). At pH 3.0-4.0 Britton-Robinson (BR) buffer medium, CS interacted with NBS to form an ion-association complex. As a result, the...
Nantana Nuchtavorn et al.
Electrophoresis, 33(9-10), 1421-1426 (2012-06-01)
Rapid detection of microorganisms by alternative methods is desirable. Electromigration separation methods have the capability to separate microorganisms according to their charge and size and laser-induced fluorescence (LIF) detection have single-cell detection capability. In this work, a new combined separation...

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