To target a carboxyl group on cellular proteins, the carbodiimide group of EDC must be unbound so the carbonyl from the carboxylic acid can initiate an attack. This interaction results in the formation of an O-acylisourea intermediate that can react with amines, with an urea by-product being released as a part of the process. Should a fluorophore be pre-conjugated to EDC, it is likely to be released as this by-product and thus would not attach to the carboxyl groups of the cells.
165344
1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide methiodide
for peptide synthesis
Synonym(s):
EDC methiodide
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About This Item
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Product Name
1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide methiodide,
form
powder
Quality Level
reaction suitability
reaction type: Coupling Reactions
mp
97-99 °C (lit.)
application(s)
peptide synthesis
functional group
amine
Storage temp.
2-8°C
SMILES string
[I-].CCN=C=NCCC[N+](C)(C)C
InChI
1S/C9H20N3.HI/c1-5-10-9-11-7-6-8-12(2,3)4;/h5-8H2,1-4H3;1H/q+1;/p-1
Inchi Key
AGSKWMRPXWHSPF-UHFFFAOYSA-M
Related Categories
Application
- Crosslinking agent
Reactant for:
- Amide bond forming (amidation) crosslinking reactions
signalword
Warning
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is it possible to have EDC conjugated with a fluorophore such that it can still target Carboxyl groups on the cellular proteins
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