MilliporeSigma
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198080

Sigma-Aldrich

Methyl Green

zinc chloride salt, certified by the Biological Stain Commission, Dye content 85 %

Synonym(s):
Ethyl Green
Linear Formula:
C27H35BrClN3 · ZnCl2
CAS Number:
Molecular Weight:
653.24
Colour Index Number:
42590
Beilstein:
3914106
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 85%

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

423 nm (2nd)
629 nm

ε (extinction coefficient)

≥11100 at 417-425 in H2O at 0.005 g/L
≥16000 at 311-319 in H2O at 0.005 g/L
≥60000 at 626-634 in H2O at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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This Item
3238291.15944M8884
Methyl Green zinc chloride salt, certified by the Biological Stain Commission, Dye content 85 %

Sigma-Aldrich

198080

Methyl Green

Methyl Green zinc chloride salt, <0.5% crystal violet, Dye content 80 %

Sigma-Aldrich

323829

Methyl Green

Methyl Green zinc chloride salt, ~85%

Sigma-Aldrich

M8884

Methyl Green

form

powder

form

solid

form

solid

form

powder

composition

Dye content, 85%

composition

Dye content, 80%

composition

Dye, ≥50% spectrophotometric assay

composition

Dye Content, ≥83%

pH

0.2-1.8, yellow to blue

pH

0.2-1.8, yellow to blue

pH

4.6 (20 °C, 10 g/L in H2O)

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL

solubility

70 g/L

solubility

water: 1 mg/mL

General description

Methyl Green is a basic dicationic dye and is composed of triphenylmethane. It is suitable for the detection of DNA and RNA. It acts as a photochromophore and used to activate gelatinous films. At a very high pH, methyl green forms an alkaline solution and gets converted to a colorless carbinol base. In the presence of hydroxide anion, methyl green produces crystal violet dye and ethanol.

Suitability

Suitable for use as a DNA stain.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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R.D. Lillie Ed.
Conn?s Biological Stains, 282-282 (1977)
Mechanistic studies of the photocatalytic degradation of methyl green: an investigation of products of the decomposition processes
Chen CC and Lu CS
Environmental Science & Technology, 41(12), 4389-4396 (2007)
Virchow?s Arch. (Pathol. Anat.), 157, 19-19 (1899)
The problem of a standardized technic for the methyl-green-pyronin stain.
E B TAFT
Stain technology, 26(3), 205-212 (1951-07-01)
Journal of the American Medical Association, 76, 240-240 (1921)

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