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199311

Sigma-Aldrich

Tetrabromophenol Blue

Dye content 75 %

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Synonym(s):
3,3′,3″,4,5,5′,5″,6-Octabromophenolsulfonphthalein
Empirical Formula (Hill Notation):
C19H6Br8O5S
CAS Number:
Molecular Weight:
985.54
Beilstein:
378438
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 75%

mp

204 °C (dec.) (lit.)

λmax

388 nm (2nd)
605 nm

ε (extinction coefficient)

≥55000 at 606-616 nm in ethanol and water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3c(Br)c(Br)c(Br)c(Br)c23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H6Br8O5S/c20-7-1-5(2-8(21)16(7)28)19(6-3-9(22)17(29)10(23)4-6)11-12(24)13(25)14(26)15(27)18(11)33(30,31)32-19/h1-4,28-29H

InChI key

QPMIVFWZGPTDPN-UHFFFAOYSA-N

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This Item
236047114405114391
Tetrabromophenol Blue Dye content 75 %

Sigma-Aldrich

199311

Tetrabromophenol Blue

Bromophenol Blue ACS reagent

Sigma-Aldrich

114391

Bromophenol Blue

composition

Dye content, 75%

composition

Dye content, 85%

composition

Dye content, 90%

composition

-

mp

204 °C (dec.) (lit.)

mp

-

mp

-

mp

273 °C (lit.)

λmax

388 nm (2nd), 605 nm

λmax

610 nm

λmax

383 nm, 589 nm

λmax

-

ε (extinction coefficient)

≥55000 at 606-616 nm in ethanol and water

ε (extinction coefficient)

≥12000 at 298-304 nm in H2O at 0.005 g/L, ≥55000 at 607-613 nm in H2O at 0.005 g/L, ≥59000 at 222-228 nm in H2O at 0.005 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Biochem/physiol Actions

Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg2(NO3)2. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes color from pale green to blue. Binding of TBPB to proteins is dependent on pH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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