The Sigma Aldrich Handbook of Stains, Dyes and Indicators advises that Lissamine Green B has been substituted for Fast Green FCF without any real advantage. Lillie found it performed well as a collagen stain in Masson's trichrome and as a counter stain in the Van Gieson method. However, it was inferior when used as a component in Van Gieson's Trichrome Stain. See also Clark G, Staining Procedures, 4th Edition. (Williams & Wilkins, Baltimore, 1981), p. 120 for the Lillie modification of Masson's trichrome method. This material is used in the determination of chlorine dioxide in solution in EPA Method 32.
199583
Lissamine™ Green B
Dye content 60 %
Synonym(s):
Acid Green 50, Wool Green S
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25 G
$75.10
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About This Item
Empirical Formula (Hill Notation):
C27H25N2NaO7S2
CAS Number:
Molecular Weight:
576.62
PubChem Substance ID:
UNSPSC Code:
12171500
Colour Index Number:
44090
NACRES:
NA.47
EC Number:
221-409-2
MDL number:
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Product Name
Lissamine™ Green B, Dye content 60 %
InChI key
WDPIZEKLJKBSOZ-UHFFFAOYSA-M
InChI
1S/C27H26N2O7S2.Na/c1-28(2)20-9-5-17(6-10-20)25(18-7-11-21(12-8-18)29(3)4)26-23-14-13-22(37(31,32)33)15-19(23)16-24(27(26)30)38(34,35)36;/h5-16H,1-4H3,(H2,31,32,33,34,35,36);/q;+1/p-1
SMILES string
[Na+].CN(C)c1ccc(cc1)C(=C2/C=CC(\C=C2)=[N+](/C)C)\c3c(O)c(cc4cc(ccc34)S([O-])(=O)=O)S([O-])(=O)=O
form
powder
composition
Dye content, 60%
solubility
H2O: 0.1%
λmax
633 nm
ε (extinction coefficient)
≥10000 at 300-306 nm in water at 0.005 g/L
≥50000 at 236-242 nm in water at 0.005 g/L
≥60000 at 630-636 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
Related Categories
1 of 4
This Item | B6756 | B4014 | L1886 |
|---|---|---|---|
| solubility H2O: 0.1% | solubility H2O: 1 mg/mL | solubility H2O: 1 mg/mL, blue | solubility H2O: 1 mg/mL |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| composition Dye content, 60% | composition Dye content, ≥85% | composition Dye content, ≥90% | composition Dye content, ≥65% |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Biochem/physiol Actions
Lissamine green also referred to as acid green 50, wool green S, food green S or green S, is used for vital staining of cornea and conjunctiva. Staining characteristics are almost similar to rose bengal dye.[3][4] Lissamine green is also used to stain membrane-damaged epithelial cells. In vitro, Lissamine green B at low concentration blocks herpes simplex virus 1 (HSV-1) plaque formation.[1]
Legal Information
Lissamine is a trademark of PerkinElmer Inc.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Photocatalytic degradation of Lissamine Green B dye by using nanostructured sol?gel TiO2 films
Curkovic L
J. Alloy Compounds, 604, 309-316 (2014)
Vascular Permeability and Microcirculation of Gliomas and Mammary Carcinomas
Transplanted in Rat and Mouse Cranial Windows1
Transplanted in Rat and Mouse Cranial Windows1
Yuan F
Cancer Research, 54, 4564- 4568 (1994)
Hannah Dueck et al.
Genome biology, 16, 122-122 (2015-06-10)
Differentiation of metazoan cells requires execution of different gene expression programs but recent single-cell transcriptome profiling has revealed considerable variation within cells of seeming identical phenotype. This brings into question the relationship between transcriptome states and cell phenotypes. Additionally, single-cell
Donald R Korb et al.
Cornea, 29(4), 377-383 (2010-02-20)
The purpose of this study was to investigate the prevalence of lid wiper epitheliopathy (LWE) in patients diagnosed with dry eye disease (DED). Patients were recruited for two groups. Inclusion criteria for the DED group (n = 50) was: a
K Y Ukhanov et al.
The Journal of general physiology, 105(1), 95-116 (1995-01-01)
Several Ca-sensitive fluorescent dyes (fura-2, mag-fura-2 and Calcium Green-5N) were used to measure intracellular calcium ion concentration, Cai, accompanying light-induced excitation of Limulus ventral nerve photoreceptors. A ratiometric procedure was developed for quantification of Calcium Green-5N fluorescence. A mixture of
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What are possible uses for Product 199583, Lissamine™ Green B?
1 answer-
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Has Product 199583, Lissamine™ Green B, been used for industrial applications?
1 answer-
The Sigma Aldrich Handbook of Stains, Dyes and Indicators states Lissamine Green B has been used to dye wool, silk (from a sulfuric acid bath), to print wool and silk directly and to coat paper, dye leather and surface stain wood.
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What is the solubility of Product 199583, Lissamine™ Green B?
1 answer-
Lissamine Green B is soluble in water up to 0.1% in water. Other sources have stated solubility for some lots can approach 4%. Solubility in ethanol is 3 mg/mL, although this is not tested by Sigma Aldrich on a lot to lot basis. Lissamine Green B has also been reported to be soluble in Cellosolve, and in ethylene glycol.
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What is the ?max 199583, Lissamine™ Green B, in water?
1 answer-
The ?max in water is 633 nm.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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