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201545

Sigma-Aldrich

Fast Blue RR

Synonym(s):

4-Benzoylamino-2,5-dimethoxyaniline, Azoic Diazo No. 24

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About This Item

Empirical Formula (Hill Notation):
C15H16N2O3
CAS Number:
Molecular Weight:
272.30
Colour Index Number:
37155
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

assay

>96.5% (HPLC)

Quality Level

form

solid

mp

165-168 °C (lit.)

λmax

323 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

COc1cc(NC(=O)c2ccccc2)c(OC)cc1N

InChI

1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)

InChI key

DDRCIGNRLHTTIW-UHFFFAOYSA-N

Related Categories

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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