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201545

Sigma-Aldrich

Fast Blue RR

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Synonym(s):
4-Benzoylamino-2,5-dimethoxyaniline, Azoic Diazo No. 24
Empirical Formula (Hill Notation):
C15H16N2O3
CAS Number:
Molecular Weight:
272.30
Colour Index Number:
37155
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Assay

>96.5% (HPLC)

Quality Level

form

solid

mp

165-168 °C (lit.)

λmax

323 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

COc1cc(NC(=O)c2ccccc2)c(OC)cc1N

InChI

1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)

InChI key

DDRCIGNRLHTTIW-UHFFFAOYSA-N

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This Item
F0125F0500FBS25
Fast Blue RR

Sigma-Aldrich

201545

Fast Blue RR

Fast Blue RR Salt crystalline

Sigma-Aldrich

F0500

Fast Blue RR Salt

Fast Blue RR Salt Preweighed Capsules

Sigma-Aldrich

FBS25

Fast Blue RR Salt

form

solid

form

powder

form

crystalline

form

powder

mp

165-168 °C (lit.)

mp

98-100 °C

mp

-

mp

-

λmax

323 nm

λmax

-

λmax

-

λmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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