During Quality Control, no testing to performed involving confocal microscopy. Conjugate 5-aminofluorescein (AFL)-human serum albumin has been used to stain brain cells, although it does not appear confocal microscopy was performed. Below is the reference: https://www.sigmaaldrich.com/tech-docs/paper/250406
201626
Fluoresceinamine, isomer I
Synonym(s):
5-Aminofluorescein
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250 MG
$66.60
1 G
$177.00
5 G
$659.00
About This Item
Empirical Formula (Hill Notation):
C20H13NO5
CAS Number:
Molecular Weight:
347.32
Beilstein/REAXYS Number:
48395
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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form
powder
Quality Level
mp
223 °C (dec.) (lit.)
solubility
methanol: 5 mg/mL
λmax
496 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChI key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
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This Item | I9271 | 46951 | F7250 |
|---|---|---|---|
| solubility methanol: 5 mg/mL | solubility DMSO: 5 mg/mL | solubility acetone: soluble 0.1 g/10 mL | solubility acetone: 1 mg/mL |
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mp 223 °C (dec.) (lit.) | mp - | mp >360 °C (lit.) | mp >360 °C (lit.) |
General description
Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.
Application
Fluoresceinamine, isomer I is suitable for use in a specific and sensitive spectrophotometric method for determining nitrite. It has been used to fluorescently tag nanoparticles through a competitive carboxyl-amine coupling reaction to visualize nanoparticle internalization.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Can this be used to stain cells and visualize them under the confocal microscope?
1 answer-
Helpful?
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Dear manager, I want to ask you 2 questions about this product. 1. Is it a flurescence dye? Can you show me its adsorption or emission spectrum? 2. Is the amine group reactive?
1 answer-
Yes, Fluoresceinamine, isomer I is classified as belonging to a group of derivatized fluorosceins. The product is fluorescent. Although no quality control testing is performed to check for excitation and emission, the dye is expected to excite around 490 nm and emit around 520 nm.
As the H2N- group is not acetylated or protonated, the amine group can be considered reactive. There is literature available stating Fluoresceinamine, isomer I can be covalently bound to human serum albumin.
Helpful?
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What is the solubility of Fluoresceinamine, isomer I, CAS 3326-34-9?
1 answer-
Soluble in methanol at a concentration of 5 mg/mL. Soluble in DMSO at a concentration of ≥ 32 mg/mL.
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