Janus Green B

certified by the Biological Stain Commission, Dye content 65 %

3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B
Empirical Formula (Hill Notation):
CAS Number:
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PubChem Substance ID:
Pricing and availability is not currently available.

Quality Level


certified by the Biological Stain Commission




Dye content, 65%


hematology: suitable
histology: suitable


395 nm
660 nm (2nd)

ε (extinction coefficient)

≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L

Featured Industry

Diagnostic Assay Manufacturing

storage temp.


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Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.


5, 25 g in glass bottle

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Intracellular insulin quantification by cell-ELISA
Andersen P and Vermette P
Experimental Cell Research, 347, 14-23 (2016)
David E. Sadava
Cell Biology: Organelle Structure and Function (1993)
R. W. Sabnis
Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications (2010)
Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method
Lee BC
Anatomical Record. Part B, New Anatomist, 286, 1-7 (2005)
Parker Lyng Andersen et al.
Experimental cell research, 347(1), 14-23 (2016-06-28)
Current methods of monitoring insulin in culture are limited to soluble insulin (secretions or lysates) or synthetic gene reporter analyses. We present an insulin-specific cell enzyme-linked immunosorbent assay (cell-ELISA) to assess relative intracellular insulin protein content of adherent cultures, normalized...

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