202029

Metanil Yellow

Dye content 70 %

• Synonym(s): 3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36
• Empirical Formula (Hill Notation): C₁₈H₁₄N₃NaO₃S
• CAS Number: 587-98-4
• Molecular Weight: 375.38
• EC Number: 209-608-2
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24852034
• MDL number: MFCD00007486
• Colour Index Number: 13065
• Beilstein/REAXYS Number: 3831568

Properties

• InChI key: NYGZLYXAPMMJTE-ANVLNOONSA-M
• InChI: 1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;
• SMILES string: [Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2
• form: solid
• composition: Dye content, 70%
• technique(s): titration: suitable
• pH: 1.5-2.7, red to yellow
• λmax: 414 nm
• ε (extinction coefficient): ≥18500 at 415.0-419.0 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 200

Description

Application

Metanil yellow has been used for staining of leukocyte granules.[1] It is also used as a counterstain in microscopic sections.[2]

Biochem/physiol Actions

Metanil yellow is an azo dye.[3] It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast.[4] Metanil yellow changes its color from red at pH 1.2 to yellow at pH 2.3.[5]

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318,H412
• pcodes: P273 - P280 - P305 + P351 + P338 - P501
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves