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202029

Sigma-Aldrich

Metanil Yellow

Dye content 70 %

Synonym(s):
Acid Yellow 36, 3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt
Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

solid

composition

Dye content, 70%

application(s)

hematology: suitable
histology: suitable
titration: suitable

pH range

1.5 - 2.7, red to yellow

λmax

414 nm

ε (extinction coefficient)

≥18500 at 415.0-419.0 nm

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Packaging

100 g in glass bottle

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast. Metanil yellow changes its color from red at pH 1.2 to yellow at pH 2.3.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 2

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Photocatalytic degradation of azo dye Metanil Yellow: Optimization and kinetic modeling using a chemometric approach
Sleiman M
Applied Catalysis. B, Environmental, 77, 1-2 (2007)
An Improved Stain for Connective Tissue Cells in Plastic-Embedded Sections
Cannon M S
Laboratory Medicine, 10, 783?785-783?785 (1979)
Cellular Localization of Latent Murine Cytomegalovirus
Koffron A J, et.al.
Journal of Virology, 72, 95-103 (1998)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et.al.
Biotechnic & Histochemistry, 66, 169-172 (1991)
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et al.
Biotechnic & Histochemistry, 66, 169-169 (1991)

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