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202029

Sigma-Aldrich

Metanil Yellow

Dye content 70 %

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Synonym(s):
3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt, Acid Yellow 36
Empirical Formula (Hill Notation):
C18H14N3NaO3S
CAS Number:
Molecular Weight:
375.38
Colour Index Number:
13065
Beilstein:
3831568
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

solid

composition

Dye content, 70%

technique(s)

titration: suitable

pH

1.5-2.7, red to yellow

λmax

414 nm

ε (extinction coefficient)

≥18500 at 415.0-419.0 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)c1cccc(c1)\N=N\c2ccc(Nc3ccccc3)cc2

InChI

1S/C18H15N3O3S.Na/c22-25(23,24)18-8-4-7-17(13-18)21-20-16-11-9-15(10-12-16)19-14-5-2-1-3-6-14;/h1-13,19H,(H,22,23,24);/q;+1/p-1/b21-20+;

InChI key

NYGZLYXAPMMJTE-ANVLNOONSA-M

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This Item
70540114510114502
Metanil Yellow Dye content 70 %

Sigma-Aldrich

202029

Metanil Yellow

Naphthol Yellow S for microscopy (Hist.), for the precipitation (of amino acids and peptides)

Sigma-Aldrich

70540

Naphthol Yellow S

Methyl Orange ACS reagent, Dye content 85 %

Sigma-Aldrich

114510

Methyl Orange

composition

Dye content, 70%

composition

-

composition

Dye content, 85%

composition

Dye content, 95%

technique(s)

titration: suitable

technique(s)

-

technique(s)

titration: suitable

technique(s)

titration: suitable

pH

1.5-2.7, red to yellow

pH

-

pH

3.0-4.4, pink to yellow

pH

-

λmax

414 nm

λmax

-

λmax

505 nm

λmax

437 nm

ε (extinction coefficient)

≥18500 at 415.0-419.0 nm

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

Application

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

Biochem/physiol Actions

Metanil yellow is an azo dye. It is used as a counterstain in PAS (periodic acid-Schiff)/iron-hematoxylin stained sections to increase contrast. Metanil yellow changes its color from red at pH 1.2 to yellow at pH 2.3.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Quintero-Hunter I, et.al.
Biotechnic & Histochemistry, 66, 169-172 (1991)
Cellular Localization of Latent Murine Cytomegalovirus
Koffron A J, et.al.
Journal of Virology, 72, 95-103 (1998)
Photocatalytic degradation of azo dye Metanil Yellow: Optimization and kinetic modeling using a chemometric approach
Sleiman M
Applied Catalysis. B, Environmental, 77, 1-2 (2007)
An Improved Stain for Connective Tissue Cells in Plastic-Embedded Sections
Cannon M S
Laboratory Medicine, 10, 783?785-783?785 (1979)
Cellular localization of latent murine cytomegalovirus.
Koffron AJ, et al.
Journal of Virology, 72, 95-95 (1998)

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