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206342

Sigma-Aldrich

Celestine blue

Dye content 80 %

Synonym(s):
Mordant Blue 14
Empirical Formula (Hill Notation):
C17H18ClN3O4
CAS Number:
Molecular Weight:
363.80
Colour Index Number:
51050
Beilstein:
3830911
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, 80%

mp

227-230 °C

solubility

water: 0.1%

λmax

642 nm

ε (extinction coefficient)

≥29000 at 640-650 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O

InChI

1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H

InChI key

VXAFJUDTZSMEFN-UHFFFAOYSA-N

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This Item
199311229121510998
Celestine blue Dye content 80 %

Sigma-Aldrich

206342

Celestine blue

Tetrabromophenol Blue Dye content 75 %

Sigma-Aldrich

199311

Tetrabromophenol Blue

Solvent Blue 59 Dye content 98 %

Sigma-Aldrich

229121

Solvent Blue 59

form

powder

form

powder

form

powder

form

powder

composition

Dye content, 80%

composition

Dye content, 75%

composition

Dye content, 98%

composition

Dye content, 95%

mp

227-230 °C

mp

204 °C (dec.) (lit.)

mp

215-217 °C (lit.)

mp

230 °C (dec.) (lit.)

solubility

water: 0.1%

solubility

-

solubility

-

solubility

ethanol: 10 mg/mL, clear

λmax

642 nm

λmax

388 nm (2nd), 605 nm

λmax

595 nm, 640 nm (2nd)

λmax

590 nm

ε (extinction coefficient)

≥29000 at 640-650 nm in water at 0.01 g/L

ε (extinction coefficient)

≥55000 at 606-616 nm in ethanol and water

ε (extinction coefficient)

-

ε (extinction coefficient)

≥15000 at 306-310 nm in 0.1 M NaOH at 0.01 g/L, ≥8000 at 378-382 nm in 0.1 M NaOH at 0.01 g/L

Application

May be substituted for hematoxylin in hematoxylin and eosin procedures.

Biochem/physiol Actions

Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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