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212407

Sigma-Aldrich

Direct Blue 71

Dye content 50 %

Empirical Formula (Hill Notation):
C40H23N7O13S4 · 4Na
CAS Number:
Molecular Weight:
1029.87
Colour Index Number:
34140
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 50%

λmax

594 nm

ε (extinction coefficient)

≥15000 at 283-289 nm in water at 0.01 g/L
≥30000 at 582-588 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Nc1ccc2c(O)c(N=Nc3ccc(N=Nc4ccc(N=Nc5cc(c6cccc(c6c5)S([O-])(=O)=O)S([O-])(=O)=O)c7ccccc47)c8ccc(cc38)S([O-])(=O)=O)c(cc2c1)S([O-])(=O)=O

InChI

1S/C40H27N7O13S4.4Na/c41-22-8-10-25-21(16-22)17-38(64(58,59)60)39(40(25)48)47-46-35-15-14-34(28-11-9-24(20-30(28)35)61(49,50)51)45-44-33-13-12-32(26-4-1-2-5-27(26)33)43-42-23-18-31-29(37(19-23)63(55,56)57)6-3-7-36(31)62(52,53)54;;;;/h1-20,48H,41H2,(H,49,50,51)(H,52,53,54)(H,55,56,57)(H,58,59,60);;;;/q;4*+1/p-4

InChI key

QTTDXDAWQMDLOF-UHFFFAOYSA-J

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This Item
D2535365548212490
Direct Blue 71 Dye content 50 %

Sigma-Aldrich

212407

Direct Blue 71

Direct Blue 15 suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

Sigma-Aldrich

D2535

Direct Blue 15

Direct Red 80 Dye content 25 %

Sigma-Aldrich

365548

Direct Red 80

Direct Red 23 Dye content 30 %

Sigma-Aldrich

212490

Direct Red 23

composition

Dye content, 50%

composition

Dye content, ~40%

composition

Dye content, 25%

composition

Dye content, 30%

λmax

594 nm

λmax

-

λmax

528 nm

λmax

507 nm

ε (extinction coefficient)

≥15000 at 283-289 nm in water at 0.01 g/L, ≥30000 at 582-588 nm in water at 0.01 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

≥12000 at 277-283 nm in H2O, ≥15000 at 223-235 nm in H2O, ≥32000 at 524-530 nm in H2O

ε (extinction coefficient)

≥15000 at 500-516 nm in water at 0.04 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Direct blue 71 is an azo dye with three azo bonds. It is useful to stain membrane-immobilized antibodies and proteins for the investigation of antigen-antibody interactions.

Application

Direct blue 71 has been used to stain sliced tissue sections mounted on the PVDF for in situ proteome analysis, and to stain the electroblotted membranes and sections of the brain to visualize the structure. Direct blue staining is also suitable for the assessment of protein binding and qualitative validation of protein concentration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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