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Sigma-Aldrich

4′,5′-Dibromofluorescein

Dye content 95 %

Synonym(s):
Solvent Red 72
Empirical Formula (Hill Notation):
C20H10Br2O5
CAS Number:
Molecular Weight:
490.10
Colour Index Number:
45370:1
Beilstein:
57189
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

>95% (TLC)

form

powder

composition

Dye content, 95%

mp

270-273 °C (lit.)

λmax

450 nm

ε (extinction coefficient)

≥5000 at 445-451 nm
≥50000 at 227-233 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

ZDTNHRWWURISAA-UHFFFAOYSA-N

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General description

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

Application

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

Packaging

5 g in glass bottle

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 3 Oral

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Potential Risk of Food-Drug Interactions: Citrus Polymethoxyflavones and Flavanones as Inhibitors of the Organic Anion Transporting Polypeptides (OATP) 1B1, 1B3, and 2B1.
Bajraktari-Sylejmani, et al.
Eur. J. Drug Metab. Pharmacokinet., 45, 809-815 (2020)
Marc Le Vée et al.
Journal of biochemical and molecular toxicology, 33(10), e22379-e22379 (2019-08-01)
The interactions of six neonicotinoid pesticides and one neonicotinoid metabolite with drug transporters have been characterized in vitro. Acetamiprid, clothianidin, imidacloprid, nitenpyram, thiacloprid and its metabolite thiacloprid amide, and thiamethoxam, each used at 100 µM, did not impair activity of the

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