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Sigma-Aldrich

4′,5′-Dibromofluorescein

Dye content 95 %

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Synonym(s):
Solvent Red 72
Empirical Formula (Hill Notation):
C20H10Br2O5
CAS Number:
Molecular Weight:
490.10
Colour Index Number:
45370:1
Beilstein:
57189
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Assay

>95% (TLC)

form

powder

composition

Dye content, 95%

mp

270-273 °C (lit.)

λmax

450 nm

ε (extinction coefficient)

≥5000 at 445-451 nm
≥50000 at 227-233 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

ZDTNHRWWURISAA-UHFFFAOYSA-N

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This Item
D6665410217491527
form

powder

form

powder

form

powder

form

-

composition

Dye content, 95%

composition

-

composition

-

composition

-

mp

270-273 °C (lit.)

mp

280 °C (dec.) (lit.)

mp

280 °C (dec.) (lit.)

mp

14-18 °C (lit.)

λmax

450 nm

λmax

-

λmax

509 nm

λmax

-

ε (extinction coefficient)

≥5000 at 445-451 nm, ≥50000 at 227-233 nm

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

General description

4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.

Application

4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 3 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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