MilliporeSigma
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227935

Sigma-Aldrich

Azure B

prepared by direct synthesis

Synonym(s):
N,N,N′-Trimethylthionin, Azure I, Methyleneazure
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein:
3922630
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

Assay

>85.0% (HPLC)

form

powder

mp

205-210 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue to very deep blue

λmax

648 nm

ε (extinction coefficient)

≥13000 at 241-247 nm
≥36000 at 287-293 nm
≥67000 at 638-644 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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This Item
A40431046351PHR2484
Azure B prepared by direct synthesis

Sigma-Aldrich

227935

Azure B

Azure B certified by the Biological Stain Commission

Sigma-Aldrich

A4043

Azure B

Azure B United States Pharmacopeia (USP) Reference Standard

USP

1046351

Azure B

Azure B Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR2484

Azure B

assay

>85.0% (HPLC)

assay

-

assay

-

assay

-

form

powder

form

powder

form

-

form

powder

mp

205-210 °C (dec.) (lit.)

mp

205-210 °C (dec.) (lit.)

mp

205-210 °C (dec.) (lit.)

mp

205-210 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue to very deep blue

solubility

H2O: 1 mg/mL

solubility

-

solubility

-

λmax

648 nm

λmax

-

λmax

-

λmax

-

ε (extinction coefficient)

≥13000 at 241-247 nm, ≥36000 at 287-293 nm, ≥67000 at 638-644 nm

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

Application

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localization of nucleic acids and lignin.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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