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230162

Sigma-Aldrich

Sulforhodamine B

Dye content 75 %

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Synonym(s):
Acid Red 52, Sulforhodamine B monosodium salt
Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein:
3886008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, 75%

solubility

methanol: 1 mg/mL

λmax

554 nm

ε (extinction coefficient)

≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

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This Item
S9012341738R6626
Sulforhodamine B Dye content 75 %

Sigma-Aldrich

230162

Sulforhodamine B

Rhodamine B ≥95% (HPLC)

Sigma-Aldrich

R6626

Rhodamine B

composition

Dye content, 75%

composition

Dye content, ~75%

composition

Dye content, 95%

composition

-

solubility

methanol: 1 mg/mL

solubility

-

solubility

-

solubility

H2O: 1 mg/mL

λmax

554 nm

λmax

-

λmax

558 nm

λmax

-

ε (extinction coefficient)

≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

104500-115800 at 542-554 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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