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235474

Sigma-Aldrich

Acridine Orange base

Dye content 75 %

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Synonym(s):
3,6-Bis(dimethylamino)acridine, Solvent Orange 15
Empirical Formula (Hill Notation):
C17H19N3
CAS Number:
Molecular Weight:
265.35
Colour Index Number:
46005
MDL number:
PubChem Substance ID:

form

powder

Quality Level

composition

Dye content, 75%

mp

165 °C (dec.) (lit.)

solubility

1 M HCl: 1 mg/mL, clear

λmax

488 nm

ε (extinction coefficient)

≥14000 at 227-233 nm
≥20000 at 289-295 nm
≥35000 at 486-492 nm
≥40000 at 266-272 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

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This Item
A6014318337158550
Acridine Orange base Dye content 75 %

Sigma-Aldrich

235474

Acridine Orange base

composition

Dye content, 75%

composition

Dye content, ~80%

composition

-

composition

Dye content, 85%

mp

165 °C (dec.) (lit.)

mp

-

mp

284-287 °C (lit.)

mp

-

solubility

1 M HCl: 1 mg/mL, clear

solubility

ethanol: 2 mg/mL, 4 mg/mL (2-methoxyethanol (EGME)), water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)

solubility

H2O: 0.1%, clear, orange to red

solubility

H2O: 1 mg/mL, clear, orange to very deep orange

λmax

488 nm

λmax

-

λmax

492 nm

λmax

489 nm

ε (extinction coefficient)

≥14000 at 227-233 nm, ≥20000 at 289-295 nm, ≥35000 at 486-492 nm, ≥40000 at 266-272 nm

ε (extinction coefficient)

-

ε (extinction coefficient)

≥14,000 at 227-233 nm in water at 0.005 g/L, ≥35000 at 265-271 nm in water at 0.005 g/L

ε (extinction coefficient)

-

General description

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.

Application

Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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