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238236

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

99%

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
Beilstein/REAXYS Number:
3912045
EC Number:
MDL number:

assay

99%

mp

258 °C (dec.) (lit.)

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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Application

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.

Certificate of Analysis

Certificate of Origin

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