302643

Sigma-Aldrich

Trypan Blue

Dye content 60 %

Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein/REAXYS Number:
4360496
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.

Quality Level

form

powder

composition

Dye content, 60%

application(s)

hematology: suitable
histology: suitable

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01  g/L
60000 at 603-607 nm in methanol

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770), PTPRF(5792)

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Related Categories

Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated anionic dye. It is also used to screen the cytotoxic properties of plant extracts. In addition, trypan blue is used for staining epiretinal membranes during vitrectomy surgery.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN 3077 9 / PGIII

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 239, 698-704 (2001)
Thangaraj Parimelazhagan
Pharmacological Assays of Plant-Based Natural Products (2013)
Role of caspase-10 in the death of acute leukemia cells
Guo W
Oncology Letters, 12, 1623-1629 (2016)
Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication
McLean J E, et.al.
Journal of Virology, 83, 8233-8246 (2009)
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic...

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