K Sasaki et al.
Bioscience, biotechnology, and biochemistry, 61(10), 1783-1785 (1997-11-15)
The absolute configuration of pectenotoxin-6 (4, PTX6), found in association with diarrhetic shellfish poisoning, was identified by NMR spectroscopy using a chiral anisotropic reagent, phenylglycine methyl ester (PGME), which was condensed with a carboxyl group of 4.
M I Youshko et al.
Biochemistry. Biokhimiia, 65(12), 1367-1375 (2001-02-15)
Kinetic regularities of the enzymatic acyl group transfer reactions have been studied using ampicillin synthesis catalyzed by E. coli penicillin acylase as an example. It was shown that ampicillin synthesis proceeds through the formation of an acylenzyme-nucleophile complex capable of...
Esther M Gabor et al.
Protein engineering, design & selection : PEDS, 17(7), 571-579 (2004-08-31)
A semi-random mutagenesis approach was followed to increase the performance of penicillin acylase PAS2 in the kinetically controlled synthesis of ampicillin from 6-aminopenicillanic acid (6-APA) and activated D-phenylglycine derivatives. We directed changes in amino acid residues to positions close to...
P S Nys et al.
Antibiotiki, 26(12), 920-932 (1981-12-01)
The possible use of various procedures for estimation of the ionization constants of the Michaelis complex by the pH dependence of the maximum enzymatic reaction rate is discussed. It is shown that the procedures described in the literature for estimation...
Anna Serra et al.
Journal of environmental monitoring : JEM, 13(1), 167-174 (2010-11-17)
A comparative Life Cycle Assessment (LCA) of solar photo-Fenton and solar photoelectro-Fenton, two solar-driven advanced oxidation processes (AOPs) devoted to the removal of non-biodegradable pollutants in water, is performed. The study is based on the removal, at laboratory scale, of...