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368881

Sigma-Aldrich

Fast Red TR Salt

Dye content 15 %

Synonym(s):

Fast Red TR Salt hemi(zinc chloride) salt, 4-Chloro-2-methylbenzenediazonium salt, Azoic Diazo No. 11

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25 G
$63.70

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25 G
$63.70

About This Item

Empirical Formula (Hill Notation):
C7H6Cl2N2 · 0.5 ZnCl2
CAS Number:
Molecular Weight:
257.19
Colour Index Number:
37085
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

$63.70


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form

powder

Quality Level

composition

Dye content, 15%

λmax

284 nm

ε (extinction coefficient)

≥3000 at 281-287 nm in water at 0.006 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc(Cl)ccc1[N+]#N.Cc2cc(Cl)ccc2[N+]#N

InChI

1S/2C7H6ClN2.4ClH.Zn/c2*1-5-4-6(8)2-3-7(5)10-9;;;;;/h2*2-4H,1H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

RIIVFDKBXXMYRL-UHFFFAOYSA-J

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General description

Fast Red TR is a diazonium dye. It serves as a chromogenic substrate for alkaline phosphatase (AP), a common antibody conjugate widely used fin immunohistochemical applications.

Application

Fast Red TR Salt hemi (zinc chloride) salt has been used in the preparation of staining solutions to stain TRACP (tartrate-resistant acid phosphatase). It is also used for the detection of alkaline phosphatase.

pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificates of Analysis (COA)

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Kessler C
Nonradioactive Labeling and Detection of Biomolecules (2012)
Effect of monoterpenes on the formation and activation of osteoclasts in vitro
Dolder S
Journal of Bone and Mineral Research, 21(4), 647-655 (2006)
Reliability of new poly (lactic-co-glycolic acid) membranes treated with oxygen plasma plus silicon dioxide layers for pre-prosthetic guided bone regeneration processes
Castillo-Dali G
Medicina Oral, patologia Oral y cirugia Bucal, 22(2), e242-e250 (2017)
T Kenzaka et al.
Microbiology (Reading, England), 144 ( Pt 8), 2085-2093 (1998-08-28)
An improved in situ hybridization technique, HNPP-FISH, using 2-hydroxy-3-naphthoic acid 2'-phenylanilide phosphate (HNPP) and Fast Red TR was applied to analyse the community structure of planktonic bacteria in river water. Oligonucleotide probes specific for the domain Bacteria (EUB338) and five
L L Chueh et al.
Journal of virological methods, 79(2), 133-140 (1999-06-25)
A sensitive fluorescence in situ hybridization (ISH) for detecting porcine reproductive and respiratory syndrome virus (PRRSV) RNA in viral infected tissue was developed using digoxigenin-labeled RNA probes targeted on the nucleocapsid gene of PRRSV. In situ RNA/RNA hybrids were detected

Questions

  1. Could you clarify what "dye content 15%" means? What makes up the remaining 85%? Does this indicate that in 100 g of powder, 15 g consists of Fast Red (MW 257.19)? I’d like to understand this better for preparing mM solutions.

    1 answer
    1. The concept of "dye content" is not rigorously defined. The term was originally defined as the percent by weight of dyes in commercial dyes which were frequently contaminated or purposely diluted with noncolored materials such as inorganic salts, solvents of crystallization, and other additives such as antidusting agents, dispersants, etc. Hence, the term refers to the percent by weight of total dyes in the mixture and should not be confused with dye purity. On a lot-to-lot basis there is no attempt made to identify the non dye constituents of product 368881. As the dye content is not expressed on the current Certificates of Analysis, there is no reasonable method for preparing molar solutions.

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