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39391

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide

≥97.0% (T), for peptide synthesis

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Synonym(s):

N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide, EDC, WSC

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Size/SKUAvailabilityPrice
10 mL
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$204.00
50 mL
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$876.00

About This Item

Empirical Formula (Hill Notation):
C8H17N3
CAS Number:
Molecular Weight:
155.24
NACRES:
NA.22
PubChem Substance ID:
UNSPSC Code:
12352116
EC Number:
217-579-2
MDL number:
Beilstein/REAXYS Number:
507429

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Product Name

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide, ≥97.0% (T)

Quality Segment

assay

≥97.0% (T)

form

liquid

reaction suitability

reaction type: Coupling Reactions

refractive index

n20/D 1.460-1.463

density

0.877 g/mL at 20 °C (lit.)

application(s)

peptide synthesis

functional group

amine

storage temp.

−20°C

SMILES string

CCN=C=NCCCN(C)C

InChI

1S/C8H17N3/c1-4-9-8-10-6-5-7-11(2)3/h4-7H2,1-3H3

InChI key

LMDZBCPBFSXMTL-UHFFFAOYSA-N

General description

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is commonly used in combination with N-hydroxysuccinimide (NHS) in carbodiimide coupling reaction to activate carboxyl group for coupling with amines to form amides.

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide has been used for the surface functionalization of graphene quantum dots (GQDs) employed as sensing probes in Janus micromotors to detect enterobacterial contamination. It has also been used for the activation of folic acid, prior to its conjugation on silica nanoparticles.

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Show Differences

1 of 1

This Item
E775042433103450
description

≥97.0% (T)

description

commercial grade, powder

description

200-400 mesh, 2 % cross-linked with divinylbenzene

description

purum, ≥98.0% (AT)

form

liquid

form

powder

form

powder

form

powder

assay

≥97.0% (T)

assay

-

assay

-

assay

≥98.0% (AT)

functional group

amine

functional group

-

functional group

-

functional group

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

200

reaction suitability

reaction type: Coupling Reactions

reaction suitability

-

reaction suitability

reaction type: solution phase peptide synthesis, reactivity: carboxyl reactive

reaction suitability

-


signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Stomach,large intestine,lymph node

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Which is the suggested EDC concentration and the relative protocol for covalently attach haptens to carrier proteins (e.g. BSA) ?

    1 answer
    1. EDC and EDAC are both abbreviations for N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide. While a detailed procedure for conjugating haptens (such as peptides) to carrier proteins such as Bovine Serum Albumin (BSA) is not available, the EDC is typically used at a 5 to 10-fold molar excess with respect to the hapten concentration in the reaction mix. Note that EDC hydrochloride (e.g., Products E6383, E7750 and 03450) is more commonly used as a coupling agent than EDC free base (Product 39391).

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. What is the concentration of the EDC chemical in the 10mL solution?

    1 answer
    1. This product is considered to be a pure/neat EDC material. It has a purity specification of 97% or greater, but it is not a solution of EDC in a solvent.

      Helpful?

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