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  • 40718
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40718

Sigma-Aldrich

Diethyl pyrocarbonate

96% (NT)

Synonym(s):
DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.22

Quality Level

Assay

96% (NT)

form

liquid

color

APHA: ≤20

refractive index

n20/D 1.398 (lit.)
n20/D 1.398

bp

93-94 °C/18 mmHg (lit.)

solubility

95% ethanol: 4.5 g/10 mL, clear, colorless

density

1.121 g/mL at 20 °C
1.101 g/mL at 25 °C (lit.)

shipped in

wet ice

storage temp.

2-8°C

SMILES string

CCOC(=O)OC(=O)OCC

InChI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

InChI key

FFYPMLJYZAEMQB-UHFFFAOYSA-N

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Diethyl pyrocarbonate 96% (NT)

Sigma-Aldrich

40718

Diethyl pyrocarbonate

Diethyl pyrocarbonate 96% (NT)

Sigma-Aldrich

159220

Diethyl pyrocarbonate

Diethyl dicarbonate for synthesis

Sigma-Aldrich

8.41751

Diethyl dicarbonate

OmniPur® DEPC

Sigma-Aldrich

3660-OP

OmniPur® DEPC

assay

96% (NT)

assay

96% (NT)

assay

≥98.0% (acidimetric)

assay

-

form

liquid

form

liquid

form

liquid

form

liquid

refractive index

n20/D 1.398 (lit.)

refractive index

n20/D 1.398 (lit.)

refractive index

-

refractive index

-

bp

93-94 °C/18 mmHg (lit.)

bp

93-94 °C/18 mmHg (lit.)

bp

93-94 °C/24 hPa

bp

-

solubility

95% ethanol: 4.5 g/10 mL, clear, colorless

solubility

-

solubility

-

solubility

-

density

1.121 g/mL at 20 °C, 1.101 g/mL at 25 °C (lit.)

density

1.101 g/mL at 25 °C (lit.)

density

1.12 g/cm3 at 20 °C

density

-

General description

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Application

Diethyl pyrocarbonate may be used in the following studies:
  • For the modification of histidyl residues in proteins.
  • As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
  • For the modification of linear and supercoiled plasmid DNAs.
  • As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
  • For cabethoxylation of histidine residues of actin.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.

Biochem/physiol Actions

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

156.2 °F - closed cup

Flash Point(C)

69 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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