739936

Sigma-Aldrich

3-epi-25-Hydroxyvitamin D3 solution

100 μg/mL in ethanol, 98% (CP)

Empirical Formula (Hill Notation):
C27H44O2
CAS Number:
Molecular Weight:
400.64
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24
Pricing and availability is not currently available.

Quality Level

assay

98% (CP)

form

solution

concentration

100 μg/mL in ethanol

application(s)

detection: suitable
mass spectrometry (MS): suitable

color

colorless

mp

-144.0 °C

storage temp.

−20°C

SMILES string

C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2\C(CCC[C@]12C)=C\C=C3\C[C@H](O)CCC3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23-,24-,25+,27-/m1/s1

InChI key

JWUBBDSIWDLEOM-UEGFJKGBSA-N

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Application

3-epi-25-Hydroxyvitamin D3 abbreviated as 3-epi-25(OH)D3 is a metabolite of vitamin D3 produced in the liver. 3-epi-25(OH)D3 is used as a biomarker to determine the status of vitamin D in the body. It can be also employed as an analytical standard for the quantification of 3-epi-25(OH)D3 molecules in human serum using the LC-MS/MS method.

Packaging

1 mL in ampule

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Codes

F

Risk Statement

11

Safety Statement

7-16

RIDADR

UN1170 - class 3 - PG 2 - Ethanol, solution

WGK Germany

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Certificate of Analysis
Certificate of Origin
Full-scan mass spectral evidence for 3-epi-25-hydroxyvitamin D3 in serum of infants and adults
Stepman HCM, et al.
Clinical Chemistry and Laboratory Medicine, 49(2), 253-256 (2011)
Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum
van den Ouweland JMW, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 967, 195-202 (2014)
Juan Le et al.
Journal of lipid research, 60(5), 1058-1064 (2019-03-25)
Vitamin D metabolite analysis possessed significant clinical value for the pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3...
Articles
This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.
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Related Content
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity
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